Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://www.twmu.ac.jp/ILA/" target="_blank">Lab HP (Japanese)</A> true 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。 Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Mbtd1 (Hemp) gene knockout mice. Exon 6, 7 and 8 (mbt-coding exons) were replaced with an IRES-GFP. Western blot analysis showed absence of Mbtb1 protein. Homozygous null mutant mice show skeletal abnormalities and are neonatal lethal. 戻し交配 系統名 (ブリーダー名) : C57BL/6N (日本チャールズリバー) 特記 : 現在のY染色体は原系統由来 開発者 / 機関 : 本田 浩章先生 / 広島大学 原爆放射線医科学研究所 B6;129P2-Mbtd1<tm1Homy> B6;129P2-Mbtd1<tm1Homy> このマウスは、Hemp遺伝子 (MGI symbol : Mbtd1, MGI ID : 2143977) のexon6の途中からexon8までをIRES-GFPで置き換えた遺伝子改変マウスであり、全身でHemp遺伝子を欠失する。 C (3-6 months) Heterozygote X Wild-type [C57BL/6NCrlCrlj] Hiroaki HONDA 本田　浩章 mouse Mbtd1 genomic DNA, jellyfish GFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 loxP Hemp KO B6-Mbtd1 KI Hemp KO B6-Mbtd1 KI 戻し交配系統名 (ブリーダー名) : C57BL/6N (日本チャールズリバー)特記 : 現在のY染色体は原系統由来 RBRC05201 C（3〜6か月） Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The Mbtd1 mutant mice were generated by replacing the mbt-coding exons with IRES-GFP and floxed neo resistant gene. The neo resistant gene was removed by microinjecting a Cre expression vector into fertilized eggs. The mutant mice were backcrossed with C57BL/6N for at least 10 times.